5.13b Hosting recombinant DNA

describe how plasmids and viruses can act as vectors, which take up pieces of DNA insert this recombinant DNA into other cells.


-recombinant DNA- mixture between 2 genes.
-transfer structure into host cell.
-remove the nuclei acid, only want protein shell.
-the plasmid are taken up by virus (recombinant DNA) to form the structure (act as a vector of the recombinant DNA)
-help transfer DNA into host cell.
-this virus is known as phage and it infects bacteria cells.
-virus attach to cell membrane of bacteria and inset the recombinant DNA into host cell.
-end of process we have bacteria cell- contains recombinant DNA and human gene for insulin
-this organism has its own DNA and the DNA from another organism known as transgenic.

5.13a Recombinant DNA

describe how plasmids and viruses can act as vectors which take up pieces of DNA then insert this recombinant DNA into other cells.


-1) plasmid- found in bacteria cells they are a ring of DNA, very small and dont carry many genes.
-2) virus- protein shell called a capsid, inside will be a nucleic acid (DNA OR RNA) the virus has no other components eg. nucleus or cytoplasm.
-3- human chromosome- length of DNA. identify gene- codes for protein insulin- hormone controlling blood sugar levels.
restriction enzyme is selected that can cut DNA.
take plasmid ring and cut it with the same restriction enzyme leaves a gap in plasmid (broken ring structure)
introduce human insulin gene to plasmid.
apply second enzyme DNA ligase which will join the DNA together.
human gene and plasmid DNA is called recombinant DNA